Riboflavin Coverage Test

Introduction

Meticulous cleaning of glassware and laboratory equipment is cruciaL for accurate scientific research and experimentation. Ensuring the absence of contaminants and residues is imperative to guarantee accurate and reliable results. To achieve this, the utilization of effective cleaning methods is essential. One way to evaluate cleaning processes, especially in intricate setups, is through the riboflavin coverage test. The test offers a reliable approach to comprehensively evaluate the thoroughness of cleaning coverage, employing the unique properties of riboflavin as a tracer substance.

Procedure

The riboflavin coverage test involves a sequential procedure aimed at evaluating the efficacy of the clean-inplace (CIP) system’s coverage:

1. Apply Riboflavin Solution
Prepare a riboflavin solution at a concentration of 0.2 g/L. Coat the vessel’s interior and glassware loaded in the washer with a misting spray bottle. This step ensures that all potentially critical surfaces are exposed to the tracer substance.

2. UV Observation (Initial)
While the riboflavin-coated surfaces are still wet, examine them under ultraviolet (UV) light. The riboflavin’s fluorescent properties make it visible under UV light, allowing easy visualization of its presence and distribution.

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