Open flame use in a Class II Biological Safety Cabinet
UPDATE! This article has been replaced by a newer, more informative version. Please read:
4 Reasons NOT to use open flames in Biosafety Cabinets
A Class II Biological Safety Cabinet (BSC) uses HEPA filtered, laminar airflow to provide operator, environmental and sample protection. For the purpose of sterility, HEPA filters are typically rated at 99.99+% efficiency for particles 0.3 micron in size.
Typical microbiological procedures often utilize Bunsen burners or other open flames to sterilize and/or reduce cross contamination; however, the use of such open flames inside of a BSC is not recommended for several reasons...
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